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Fig. 8. Cytosol induces clathrin to exchange gradually on the coated-pits. (A) permeabilized GFP-clathrin cells (a,c,e,g) were incubated with cytosol (2 mg/ml) for the indicated times, fixed and then stained with X22, the anti-clathrin antibody (b,d,f,h). Notice that, all fluorescence settings were held constant when comparing samples. Bars, 5 µm. (B) Distribution of uncoating time obtained from 200 puncta (five cells) uncoated by cytosol. Measured puncta were no larger than 0.3 µm.
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