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Fig. 1. Enriched oligodendrocyte cultures form MLMs even in the absence of neurons. (A) Primary cultures of oligodendrocytes with membrane sheets at an early stage of differentiation were stained for MBP and PLP. (B) Immunofluorescence analysis of the same culture at a later stage of differentiation. Notice the many thin processes, which contain pearl-like membrane patches (arrows). These patches are recognized by antibodies directed at both major myelin proteins MBP and PLP. Membrane patches also contain the myelin lipids cholesterol (C-E), galactosylceramide (C) and sulfatide (E), as shown by reactivity with filipin (C-E) and antibodies against the O1 (C) and O4 (E) epitopes. (F) Electron micrograph of differentiated oligodendrocyte shows multiple thin processes containing MLMs. (G,H) Multilamellar membranes at different stages of compaction. MLMs were seen in almost all of the cells. The interior of these structures were most often electron lucent and did not contain axons. Bar, 20 µm or as indicated.
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