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Fig. 7. Localization of Arp2/3-complex mRNA is dependent on both actin filaments and microtubules. Cytochalasin D (CD) and colchicine were used to disrupt actin filaments and microtubules in the fibroblasts, respectively. (A,C,E,G) Cells treated with 0.4 µM cytochalasin D; (B,D,F,H) cells treated with 5 µM colchicine. (A,B) Cells stained for actin filaments with fluorescein-phalloidin. (C,D) Cells stained for microtubules with mouse anti-{alpha}-tubulin antibody. (E,F) Localization of ß-actin mRNA is dependent on actin filaments but not on microtubules. By contrast, localization of Arp2/3-complex mRNA is dependent on both actin filaments and microtubules (represented by Arp3 mRNA in G,H).





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