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Fig. 5. Dynamics of [Ca2+]i and cell volume. Individual traces of the relative volume (dashed) and Fluo-4 fluorescence intensity (solid) of single cells exposed to hypotonic conditions at t=0 seconds. Relative values are expressed as ratios to control conditions (t≤0 seconds). (A-C) HaCaT cells. (D-F) EGFP-TRPV4-transfected CHO cells. Notice that elevation of [Ca2+]i follows cell swelling in both cell models. The duration of the [Ca2+]i peak is in general much shorter in transfected CHO cells than in HaCaT cells, in which [Ca2+]i is at least elevated throughout the maximum swelling phase.





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