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Fig. 2. High-level of re-engraftment in animals following SP tail vein injection. Bone marrow from the female mice that had undergone lethal irradiation followed by tail vein injection of 104 SP cells isolated from ROSA26 bone marrow was incubated with FDG to assess the level of engraftment of the bone marrow by the male donor cells. The levels of engraftment were assessed 3 months post SP cell infusion and 7 days post tracheal instillation with 2% polidocanol. Levels of engraftment were calculated as the number of cells that were able to convert FDG to its fluorescent product and could be measured by flow cytometry analysis. (A) Both the positive (ROSA26) and negative (CBA/Ca) bone marrow controls were found to contain 72.98% and 3.3% fluorescent cells respectively. Bone marrow from the experimental mice (SP1-SP5) were found to contain similar levels of fluorescent cells (mean 72.68%) to the positive ROSA26 bone marrow control demonstrating high-levels of bone marrow engraftment. (B) Flow cytometry profiles show the shift in fluorescence obtained from the bone marrow of mice SP1 to SP5 compared with the control samples.





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