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Fig. 3. PCR analysis of embryos derived from SP injected blastocysts. Cells from C57Bl/6 (WT) mice and ROSA26 (R) mice were mixed at different concentrations, DNA extracted and PCRs performed to assess the ability to detect LacZ DNA by PCR. The resulting gel showed that the ability to detect LacZ DNA is lost at greater than 1:1000 dilution with wild-type (WT) DNA (A). DNA was prepared from tissue obtained from embryos (a-g) that resulted from blastocyst injections with ROSA26 bone marrow SP cells and analysed by PCR for the presence of LacZ DNA and WT DNA. DNA was extracted from embryonic tissue that appeared to show blue X-gal positive staining. PCR controls are shown in panel (B). The resulting gels showed no presence of LacZ DNA (C) whereas WT DNA was detected (D). C, water control; M, molecular mass marker; R, ROSA26 DNA; WT, wild-type DNA.
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