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Fig. 7. No X-gal positive cells in tracheas of mice transplanted with ROSA26 bone marrow-derived SP Cells. X-gal staining was used to detect the presence of ROSA26 donor-derived cells, which could be visualised as blue cells as shown in ROSA26 tissue (A,B). The presence of blue X-gal stained ROSA26 bone marrow-derived donor cells was not detected in the tracheas of tissues removed from the mice transplanted with the ROSA26 bone marrow-derived SP cells (C,D). (A-D) Bar, 50 µM. LacZ DNA was detected by PCR in DNA extracted from tracheal section of the five ROSA26 SP transplanted mice (SP1-SP5) (E). Positive Y chromosome PCR of the DNA extracted from the tracheal sections confirmed the presence of male donor cells (F). C, water control; M, molecular mass marker; R, ROSA26 DNA; WT, wild-type DNA.
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