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Fig. 9. Cytokeratin staining and Y chromosome FISH. The Y chromosome positive donor cells (TxRd; red) that were detected in the tracheas of the mice that had undergone transplantation with bone marrow-derived SP cells were immunostained for cytokeratin expression (FITC; green). Photomicrographs (A,B) show Y chromosome FISH staining of a male trachea followed by immunohistochemistry to detect cytokeratin expression on the trachea which is normally widespread. Image C shows a Y chromosome positive donor cell that is found on the tracheal epithelium that does not express cytokeratin. Photomicrographs (D-F) show the DAPI, FITC and TxRd images of micrograph C highlighting the lack of cytokeratin staining on the donor cell. (G-J) shows a donor cell that is cytokeratin positive (arrowhead) and one that appears to be cytokeratin negative (arrow) with photomicrographs (H-J) showing DAPI, FITC and TxRd images respectively of micrograph G. (K) Confocal image of Y chromosome and pan cytokeratin positive cells (arrowheads). Bars, 50 µM (A,B); 5 µM (C-K).





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