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Fig. 10. KIF13B inhibits the ARF6 GAP activity of centaurin-{alpha}1. HeLa cells were transfected with HA-ARF6, GFP or GFP-centaurin-{alpha}1, and Myc-KIF13B{Delta} or Myc-KIF13B. After 2 days, the cells were serum starved for 2 hours, incubated with or without EGF (200 ng/ml) for 5 minutes and fixed with paraformaldehyde. The fixed cells were then immunostained using anti-HA monoclonal and anti-Myc polyclonal primary antibodies, and Cy3- and Cy5-labelled secondary antibodies. The immunostained cells were imaged by confocal microscopy. The images are representative of 80-90% of 40-50 transfected cells from five similar experiments. Arrows indicate regions within the cell where KIF13B is localised. Magnification 100 x.





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