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Fig. 4. Centaurin-{alpha}1 directly binds KIF13B in vitro. (A) 35S-radiolabelled KIF13B{Delta}C (aa 1-991) was expressed using an in vitro translation system and incubated with glutathione beads immobilized with either GST or GST-centaurin-{alpha}1. The beads were washed and bound protein was resolved by SDS-PAGE and detected by fluorography. (B,C) Purified recombinant centaurin-{alpha}1 was incubated with beads linked to GST or GST-KIF13B{Delta}. After washing the beads, protein bound to the beads was resolved by SDS-PAGE and analysed by Coomassie Blue staining (B) or immunostaining using an anti-centaurin-{alpha}1 antibody (C). The experiment was repeated two further times with identical results.





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