|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 2. (A) Surface expression of receptor GCs as shown by binding of biotinylated CNP in non-transfected and VILIP-1-transfected C6 cells. Non-transfected (C6) and VILIP-1-transfected (C6-V1) C6 cells were incubated with biotinylated CNP (0.2 µM for 20 seconds) and the relative surface expression of CNP-bound GC was measured using a streptavidin ELISA assay. (B) Time course of surface expression of GC receptors following ligand stimulation with biotinylated CNP in non-transfected and VILIP-1-transfected C6 cells. Non-transfected (C6) and VILIP-1-transfected (C6-V1) C6 cells were incubated with biotinylated CNP (0.2 µM) for a time period of 1, 5, 10, 15, 20 and 30 minutes and the surface expression of CNP-bound GC was measured in a streptavidin ELISA. Values were normalized to the 1 minutes value in C6 and C6-V1 cells, respectively. Mean values±s.e.m. are from five experiments in A and three in B. Asterisks (*,***) mark significant differences (P<0.05 with n=3, P<0.005 with n=5, Student's t-test).
|
