QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 4. Co-localization of GC-B and endogenous VILIP-1 in hippocampal neurons. Hippocampal cell cultures (14 days in vitro) were labelled by indirect immunofluorescence using affinity-purified rat and rabbit polyclonal antibodies against VILIP-1 and GC-B, monoclonal MAP-2 and secondary Cy3 antibodies (red) or secondary Alexa Fluor^TM 488 antibodies (green). (A) Co-localization of VILIP-1 (red) and MAP-2 (green). (B) Co-localization of GC-B (red) and MAP-2 (green). Note, some MAP-2-positive neurons in A and B do not express VILIP-1 or GC-B (arrows). (C) Co-localization of VILIP-1 (green) and GC-B (red). (D) Magnification of hippocampal neurons in C expressing GC-B (red). (E) Magnification of hippocampal neurons in C expressing VILIP-1 (green). (F) Merged images of D and E show co-localization of GC-B and VILIP-1 (yellow) at higher magnification. (G) Localization of GC-B (red) in dendrites of a hippocampal neuron. (H) Localization of VILIP-1 (green) in dendrites of a hippocampal neuron. (I) Merged images of G and H show co-localization (yellow) of GC-B with VILIP-1 in hippocampal dendrites. Insets in D and E show background staining with secondary antibodies only. Bars in A=30 µm, in D=20 µm and in G=5 µm.

Return to article