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Fig. 4. Less PfEMP1 is expressed on the surface of K– IE. Western blot analysis of whole cell extracts isolated from K+ and K– trophozoite IEs expressing type 41 PfEMP1. Size-fractionated extracts were hybridised with antibodies to either the conserved C-terminus of type 41 PfEMP1 ({alpha}-ATS), Exp1, aldolase or KAHRP. Fractions were also isolated from trypsin-treated (5 minutes in 100 µg ml–1) IEs. The mean and range of three densitometry measurements of the {alpha}-ATS signal are indicated. Equal signal intensities to KAHRP, Exp-1 and aldolase confirm equal loading of protein extracts and the lack of KAHRP expression in K– IEs. The molecular weights of proteins labelled are indicated (kDa).





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