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Fig. 5. PfEMP1 on the surface of K+ and K– IEs appears to share a punctate and static distribution. (A) IFA of either unfixed (mAbBC6 and {alpha}-CD59) or acetone-fixed ({alpha}-KAHRP) type 41 PfEMP1-expressing K+ and K– IE. Binding of antibody reagents to IEs was detected using FITC-conjugated IgG (green) in the presence of the nuclear DAPI stain (blue). For each image the corresponding bright field image is shown. A Z-plane towards the centre of an IE is shown. Bar, 5 µm. (B) IFA of mAbBC6 stained type 41 PfEMP1-expressing K+ and K– IEs. The Z-plane has been selected to show the exterior face of the IE. Time-lapse photographs (0, 3 and 6 minutes) of the fluorescence signals are shown. Bar, 5 µm.





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