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Fig. 2. Effect of mutations in the mouse leptin receptor on signalling. Hek293T cells were transiently co-transfected with pMET7-mLRlo_BglII plasmids encoding different leptin receptor (LR) mutants and the pXP2d2-rPAP1-luci reporter construct. The transfected cells were either stimulated with leptin for 24 hours or left unstimulated. Luciferase measurements were performed in duplicate. Results are shown as fold inductions, i.e. the luciferase signal of stimulated cells divided by the signal of unstimulated cells. Data are representative of five separate transfection experiments. As positive control, wild-type pMET7-mLRlo_BglII was used; as negative control, an empty vector was transfected. The bracket groups the six mutants with the most drastic effect on signalling: I501A, F502A, L503A, L504A, S505A and D615A.
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