|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 7. Depletion of CAP-D2 and DNA topoisomerase II by double RNAi in S2 cells. (A) The depletion of CAP-D2 and topo II after treatment with two dsRNAs was assessed at 72 and 96 hours by immunoblot analysis. Both proteins were similarly depleted and almost undetectable by 96 hours. 
-tubulin was used as a loading control. –, no dsRNA; C, control dsRNA; D, CAP-D2 dsRNA; T, topoII dsRNA; D/T, CAP-D2/topo II dsRNA. (B,C) Analysis of chromosome morphology in control (B) and CAP-D2/Topo II doubly-depleted (C) cells. Cells were cytospun onto poly-L-lysine slides, extracted during fixation and stained for topo II (red), CAP-D2 (green), CID (white) and DNA (blue), 96 hours after dsRNA treatment. Chromosome morphology was abnormal and remarkably similar to that observed after single CAP-D2 RNAi. Centromeres were stretched along the chromatin in anaphase as observed after CAP-D2 depletion alone. Scale bar: 10 µm.
|
