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Fig. 1. Matrix organization of AECs. AECs at 2 and 4 days after isolation were processed for double-label indirect immunofluorescence microscopy using antibodies against perlecan (A,D) in combination with an antiserum against the ß1 laminin subunit (B,E). Images of cells were generated using confocal laser scanning microscopy. The focal plane was as close as possible to the substratum-attached surface of the cells. Areas of co-localization are indicated by the yellow color in the overlay images shown in C,F. Bar, 10 µm.





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