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Fig. 6. Laminin fibers in the matrix of AECs undergo deformation upon stretching. (A) The 
3 laminin subunit in native matrix of AECs maintained on an elastomer membrane was immunostained and then the membrane was mounted on the stage of a fluorescent microscope. The membrane was then subjected to cyclic stretching for 10 minutes and then allowed to relax. Images of matrix were captured before, during and after stretching, and colorized in green, red and blue, respectively, as indicated in A. An overlay of the color images is shown. (B) Graphical representation of the changes in area enclosed by the matrix fiber network and fiber length during stretching and following stretching (relaxed). Each measurement is relative to that of unstretched matrix and represents eight different lengths and areas in three independent trials. *, P<0.001. Bar, 10 µm (A).
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