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Fig. 1. Cdc42 controls Golgi reorientation and the polarized localization of Rac-dependent protrusions. (A) A representative cell injected with pcDNA3::GFP-ß-actin (Actin) and pRFP-F (Membrane) along with either control pRK5-MYC or pRK5-MYC::WASp CRIB. Expression of MYC-WASp-CRIB was confirmed by immunofluorescence (not shown). The front edge of the scratched monolayer is visualized with phalloidin staining (not shown) and is indicated on the merged image as a white line. Protrusions, identified by their typical lamellipodium-like, smooth convex shape, are normally found at the front (open arrows), but upon inhibition of Cdc42 by WASp-CRIB expression, protrusions are depolarized and can now be seen at the back (filled arrows). Insets are magnified (2x) views of the boxed areas. (B) The effects of various constructs on Golgi reorientation (black bars) or protrusion polarization (white bars). Error bars represent standard deviation. Asterisks mark significant (*P<0.01) and highly significant (***P<0.0001) differences compared with control. (C) Representative cell co-injected with pEGFP::WASp-CRIB, pRFP-F and pRK5-MYC::RacN17. The cell shows no typical protrusions. Bars, 20 µm.





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