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Fig. 2. The Par6/aPKC pathway controls Golgi reorientation but not localization of protrusions. (A) A representative cell injected with pEGFP-F (membrane) and either pMT21::PKC{zeta} kinase dead (KD) or pRK5-MYC::Par6 Nt. Expression was checked by immunofluorescence (not shown). Protrusions are mainly found at the front (open arrows), while Golgi (asterisk) reorientation into the 120° sector facing the wound is inhibited. (B) Effects of various constructs on Golgi reorientation (black bars) or protrusion polarization (white bars). Error bars represent standard deviation. Asterisks mark significant (*P<0.01) and highly significant (**P<0.001) differences compared with control. Bars, 20 µm.





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