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Fig. 6. Pak activity controls the specific localization of ßPIX to the leading edge. Pak activity is needed to sequester ßPIX at the front. Endogenous PIX is normally found in focal-complex-like puncta (filled arrowheads) located at the front in control-injected cells (top panel). Inhibition of Pak by injection of pEGFP::Pak1 AID (PakAID) delocalizes PIX throughout the cell [bottom panel; at the front (filled arrowheads) and also at the back (open arrowheads)]. Bars, 20 µm.





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