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Fig. 6. ß1 integrin-deficient cells show impaired migration on both fibronectin and laminin-1. Intact neurospheres were plated and cultured in the absence or presence of exogenous growth factors on either fibronectin (FN), laminin-1 (LM1) or PDL. After 5 days, cell migration from individual mutant and control neurospheres was measured as described in Materials and Methods. (A) A significant decrease in the migration capacity of mutant cells is found on both fibronectin and laminin-1, whereas no difference is detected on PDL. Addition of growth factors (3.3 ng/ml EGF, 2.0 ng/ml FGF, 10 ng/ml NGF) partially restores the migration capacity of neurosphere mutant cells on laminin, but not on fibronectin. Note that the increase in the number of migrating cells in the presence of added growth factors does not reflect an increase in the cell migration index (see Materials and Methods). Results are the mean migration index±s.d. of three separate experiments; **P<0.01, ***P<0.001 when compared with the index in the relevant control cells. (B-M) Representative images of cells migrating from neurospheres cultured in the absence of growth factors (B-G) and in the presence of growth factors (H-M). Note the striking absence of migrating cells in the mutants plated on fibronectin (E) and the increase in migration in the mutants cultured with growth factors (L) on laminin-1 compared with the same genotype in the absence of growth factors (F). Bar, 200 µm.
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