JCS02380 Supplementary Material

Files in this Data Supplement:

• Movie 1 -

  Movie 1. REF52 cells treated with the control (luciferase) siRNA migrating into a wound. Confluent REF52 cells were artificially wounded with a pipette tip three days following siRNA transfection. One hour after wounding, images were collected every 5 minutes for 7 hours. See Fig. 6A for the corresponding still images.

• Movie 2 -

  Movie 2. REF52 cells treated with the FAK siRNA migrating into a wound. Confluent REF52 cells were artificially wounded with a pipette tip three days following siRNA transfection. One hour after wounding, images were collected every 5 minutes for 7 hours. See Fig. 6B for the corresponding still images.

• Movie 3 -

  Movie 3. Rat-2 cells treated with the control (luciferase) siRNA undergoing spontaneous polarization after being plated in sparse conditions. Ten minutes after plating, images were collected every 2 minutes for 4 hours. See Fig. 7A for the corresponding still images.

• Movie 4 -

  Movie 4. Rat-2 cells treated with the FAK siRNA fail to spontaneously polarize when plated in sparse conditions. Ten minutes after plating, images were collected every 2 minutes for 4 hours. See Fig. 7B for the corresponding still images.

• Movie 5 -

  Movie 5. Primary wild-type mouse embryo fibroblasts migrating into a wound. One hour after the confluent monolayer was wounded with a pipette tip, images were collected every 5 minutes for 7 hours. See Fig. 9C for the corresponding still images.

• Movie 6 -

  Movie 6. Primary FAK-Flox mouse embryo fibroblasts (following Cre-mediated excision of FAK) migrating into a wound. One hour after the confluent monolayer was wounded with a pipette tip, images were collected every 5 minutes for 7 hours. See Fig. 9D for the corresponding still images.