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Fig. 5. The projections in the cells treated with FAK siRNAs are rich in cortactin and are dependent on functional Rac. Cells treated with either luciferase siRNA (A) or FAK siRNA (B-F) were allowed to spread on fibronectin for 1 hour. (A) Control cells stained for cortactin. (B) FAK knockdown cells stained for cortactin. Arrowheads indicate cortactin staining along the cell periphery in A and in the multiple cellular extensions in B. (C) Cortactin staining in FAK knockdown cells following re-expression of GFP-wtFAK. Arrow indicates cell transfected with GFP-wtFAK. D is the corresponding GFP image for C. (E-F) FAK knockdown cells expressing GFP-dnRac. E shows the GFP image and F shows the corresponding phase image. Arrows point to cells expressing GFP-dnRac.





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