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Fig. 7. Treatment of Rat-2 cells with FAK siRNAs inhibits spontaneous polarization. Rat-2 cells were either transfected with luciferase siRNA (A) or FAK siRNA (B) and allowed to spread on fibronectin for 10 minutes before video time-lapse microscopy. Images were taken at the indicated timepoints after the initiation of the recording. The arrows in A indicate the broad lamellipodia at the leading edge of the migrating cells; the arrowheads in B indicate the multiple extensions that form in the FAK knockdown cells. See Movies 3 and 4 in supplementary material.