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Fig. 10. The F-actin structures that accumulate in Alix-depleted cells are not accessible to fluid phase markers. HeLa cells were treated with control RNA (A) or with siRNA (B) against Alix (see Fig. 1B). The cells were left to internalise biotinylated dextran (5 mg/ml) for 30 minutes at 37°C, and then washed once with medium at 37°C. The cells were permeabilised before fixation on ice and stained for confocal microscopy using FITC-streptavidin (green) and rhodamine-phalloidin (red). Scale bars: 5 µm.
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