|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 3. The Cabni1
mutant exhibited morphological defects during both yeast and hyphal growth. The yeast cells (A) of the wild-type (CaBNI1, strain WYL4) and the mutant (Cabni1, strain WYL3) were grown in YPD at 30°C to exponential phase. The hyphal cells (B) were induced in YPD containing 10% serum at 37°C for 2 and 4 hours. (C) Cabni1 mutant exhibited random budding. The bud scars were visualized by Calcofluor staining. (D) Cells were grown on solid medium containing 0.05 mM ammonium sulphate for 3 days at 37°C. (E) Strains WYL23 (Cabnr1 CaBNI1) and WYL25 (Cabnr1 pMET3CaBNI1) were streaked onto two GMM plates, one containing 2.5 mM each of methionine (Met) and cysteine (Cys) and the other not. The plates were incubated at 30°C for 3 days. (F) WYL25 yeast cells were inoculated into liquid GMM supplemented with or without 2.5 mM methionine and cysteine and grown at 30°C for 5 hours (top). Then serum was added to each culture to a final concentration of 10% and the cells were incubated at 37°C for 3 hours (bottom). Bars, 5 µm.
|
