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Fig. 4. Cabni1{Delta} cells showed actin patch mislocalization. The exponentially growing yeast (A) and hyphal (B) cells of the CaBNI1 (WYL4) and Cabni1{Delta} (WYL3) were stained using rhodamine-phalloidin to visualize actin. The arrows indicate actin cables. (C) Localization of GFP-CaBnr1p (strain WYL19) and CaSpa2p-GFP (strain WYL20) in Cabni1{Delta} yeast (top) and hyphal (bottom) cells. Though the signals were faint, GFP-CaBnr1p was unambiguously detected at the bud neck but never seen at the bud tip. The arrows indicate GFP-CaBnr1p localization at the neck. The pattern of CaSpa2p-GFP localization is representative of CaBud6p-GFP (not shown). (D) Actin staining. Strain WYL25 (Cabnr1{Delta} pMET3-CaBNI1) yeast cells were grown in GMM at 30°C to log phase (a) or grown in GMM containing 2.5 mM each of methionine and cysteine at 30°C for 5 hours (b); 10% serum was added to the culture shown in b for hyphal induction at 37°C for 3 hours (c). Bars, 5 µm.





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