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Fig. 1. Basigin gene structure and developmental northern blots. (A) Structure of the bsg gene encodes nine transcripts according to data from the Drosophila genome project. Transcribed portions of the gene are shown as blue boxes. Transcripts fall into classes 1a-d, 2a-d and 3. Transcripts 1a-d and 2a-d encode the same protein, D-basigin 265 (sequence shown in Fig. 2). Transcript 3 encodes a slightly longer protein, D-basigin 298. The P elements, P1096 and P1478, inserted at precisely the same location (marked by arrow) 1145 bp from the ATG for D-basigin 265 and 981 bp from the start of the first coding exon. The location of the start codon for transcript 3 is also indicated. The excision line 19 (black bar) contains a 4 kb deletion that removes the first coding exon. (B) Northern blot showing expression of bsg in embryos at stages 5, 9, 13, and 16, first-, second- and third-instar larvae and adult heads (A). The major band of
2.5 kb is consistent with the length of class 1 and 2 bsg transcripts.