QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 5. D-basigin partially colocalizes with integrin in the visual system. (A) Frozen section of retina (re) and lamina (la, bracket) of fly heterozygous for P1096 enhancer trap. This expresses nuclear ß-gal and is labeled with anti-ß-gal and visualized with Vector ABC kit. Expression was seen in the nuclei of photoreceptor neurons R1-6 (downward pointing arrow; these nuclei occur in a tight distal cluster in each ommatidium) and R7 (upward pointing arrow) and in the migratory retinal basal glia (RBG, arrowhead) (Choi and Benzer, 1994). The RBG lie just beneath the retinal basement membrane. (B) White-eyed animal labeled with anti-D-basigin and visualized with Alexa 568-conjugated secondary antibody. (C) The same section labeled with anti-ßPS1 integrin monoclonal antibody, visualized with Alexa 488-conjugated secondary antibody. D-basigin and integrin colocalize to several places, see arrowheads. However, integrin is expressed in a thin line at the basement membrane, which lacks D-basigin expression (arrows in C). Bar, 25 µm.

Return to article