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Fig. 3. Activated RhoB induces the assembly of an F-actin coat around early endosomes, which appears associated to actin filaments. (A) High magnification microscopical analysis of endosomes of cells expressing myc-RhoBwt (a-c) or myc-RhoB^G14V (d-f) and stained for Myc, EEA1 and F-actin. Both RhoBwt and RhoB^G14V localised to EEA1-positive endosomes (a,b and d,e). RhoBwt-positive endosomes showed polymerized actin on a discrete site on their membranes (arrows in a-c and merge). RhoB^G14V-positive endosomes were coated with F-actin and apparently associated with actin cables (arrows in d-f and merge). Bars: 2.5 µm. (B) Cells expressing GFP-actin were microinjected with myc-RhoB^G14V plasmids and stained for Myc. High magnification images of the RhoB^G14V-positive vesicles (indicated by arrowheads) show that these vesicles were coated with actin and apparently associated with actin fibres. Bar: 10 µm.

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