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Fig. 3. klp67A mutants mislocalise the central spindle-dependent proteins Pav-KLP, KLP3A and Asp. Microtubules are in green and DNA is shown in blue. (A) In control cells (inset) pavarotti-kinesin like protein (Pav-KLP; red) assumes a band-like distribution on the overlapping and interdigitating plus ends of central spindle microtubules. Loss of klp67A prevents the formation of this homogeneous band, but Pav-KLP still associates with the variable numbers of central spindle microtubule bundles that form, which in severe cases is very few, where it appears as aggregates (arrow). (B) Like Pav-KLP, KLP3A (red) localises to the midzone of wild-type central spindles and midbodies (inset). In klp67A mutants the motor protein is detected as ragged streaks that correspond to the poorly formed central spindle microtubule bundles. (C) Asp (red) associates with microtubule minus ends. In wild-type cells (inset) Asp localises to the centrosomes as well as the ends of the microtubules that have been released from the centrosomes and spindle poles to form the central spindle. Similarly, in mutants, Asp associates with the centrosomes and at the termini of the central spindle microtubules (arrowheads). As indicated by the arrows, irregularly spaced Asp signals are found near the equators of klp67A mutant cells, indicative of variable microtubule lengths or positions. Bars, 10 µm.
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