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Fig. 7. klp67A mutants fail to form continuous actin and anillin rings. Wild-type (insets) and klp67A mutants were fixed and stained for actin (A; red) or anillin (B; red). Microtubules are shown in green and DNA in blue. In contrast to wild-type cells, which form continuous rings of actin at their equators, actin staining is often disorganised or in streaks in the mutants and in some instances is found primarily as cortically associated foci. Similarly, anillin staining is aberrant in mutants (B) and is patchy or discontinuous. The extent to which either actin or anillin form homogenous bands correlates with the integrity of the central spindle, with the proteins being more symmetrically distributed when robust microtubule bundles are present. Bars, 10 µm.
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