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Fig. 1. Localization of claudin-7 in porcine (A) and rat (B) kidney cortex. Frozen sections of kidney cortex were immunostained with anti-claudin-7 antibody and tubular markers Claudin-7 was found to be localized in epithelial cells of the distal and collecting tubules using the distal tubular marker TSC (a and c) and the collecting tubular marker NCX1 (e and f). Because all the antibodies used were rabbit polyclonal antibodies the images in a and c, and in g and i are from adjacent sections (b and d, and h and j are the corresponding phase-contrast images). Images in e and f are from the same section with double immunolabeling. (A) In porcine kidney, claudin-7 is present in the apical cell junctions (arrows in a and e). Claudin-7 was also present in a punctate pattern at the thick ascending limb of Henle (TALH), identified by BSC-1 (asterisks and arrows in g and i). The junctional staining of claudin-7 (indicated by two arrowheads in g) was not in the TALH as it was not co-localized with the BSC-1 staining (two arrowheads in i). L: lumen of the tubule. (B) In rat kidney, Claudin-7 was present in the distal tubule (a), collecting tubule (e) and TALH (g). Arrows in a, e, and g indicated the claudin-7 signal at the apical surface of tubules. Scale bar: 30 µm (A) and 20 µm (B).
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