spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 3. Immunoblot analysis of endogenous tight junction proteins in parental, GFP-transfected, and claudin-7-GFP-transfected cells (mixed clones). (A) Parental (P), GFP-transfected (G), and claudin-7-GFP-transfected (7) cells were lysed in RIPA buffer, and a total of 20 µg protein for each lane was loaded onto the SDS-polyacrylamide gel. Membranes were blotted against claudin-1, -2, -3, -4, -7 and ZO-1 antibodies. Actin staining was used as a loading control. (B) Densitometry analysis of protein expression levels. Following immunoblotting, X-ray films were scanned and band images were analyzed. The relative signal intensity of each band was obtained after background subtraction. The band intensity for parental cells was normalized to 1 and set as the reference. Data were collected from three independent clones with high expression levels of GFP and claudin-7-GFP. The average density values for three separate experiments were plotted for each designated claudin protein.





Right arrow Return to article