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Fig. 4. Claudin-7 overexpression resulted in an increased TER in culture medium and P1 buffer. (A) LLC-PK1 cells were plated on Transwell plates and cultured for 7 days. TER was measured in the culture medium and determined as described in Materials and Methods. Claudin-7-overexpressing cells (2) showed significantly higher TER (P<0.001) than that of control cells (1). (B) TER was measured in P1 buffer containing 140 mM NaCl. Cells overexpressing claudin-7 (2) had a significant increase (P<0.01) in TER compared to that of control cells (1). Data are represented as means ± s.e.m. from at least three mixed clones with 24 monolayers (n=24) for each experiment. Student's t-test was used for statistical analysis.





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