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Fig. 1. Identification of BPGAP1-interacting partner, EEN/endophilin II via protein precipitation and MALDI-TOF. (A) Full-length BPGAP1 expressed as GST-recombinant coupled to glutathione beads were incubated with either lysis buffer (–) or 293T cell lysates (+) that had been pre-cleared with GST-beads to remove non-specific binding. Bound proteins were resolved by SDS-PAGE and revealed by silver-staining. M, molecular weight marker in kilodalton (kDa). A unique band at 46 kDa (indicated by an arrow) was subjected to trypsin digestion followed by MALDI-TOF analyses as described in Materials and Methods. (B) Protein sequence of EEN with sequence coverage of 34% (underlined) over the protein.





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