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Fig. 1. Specificity of incorporation of fluorescent tubulin in different MT arrays of unpolarised zygotes. Zygotes are positioned with the light coming from the top of the page and were microinjected on the left side. (A) Oospheres 4 hours after injection (1 optical section). (B) Cortical area of a 5 hours zygote (projection of the 2 upper optical sections): MTs are homogeneously distributed and randomly oriented. (C) One 15 µm deep section shows the cytoplasmic MT array in a 6-hour-old zygote. (D) Projection of section from 30 to 38 µm deep nuclear MT array in a 6-hour-old zygote. (E-L). Effect of nocodazole on MT arrays. (E-H) 7-hour-old zygote before and (I-L) after 2 hours incubation with nocodazole. (E,I) Cortical MTs showing only a few MTs and fluorescent foci (free non incorporated tubulin) remaining after nocodazole treatment. (F) Cytoplasmic region (projection from 7.5 to 27 µm deep inside the zygote) showing a dense cortical MT array at the periphery of the zygote. After incubation with nocodazole (J) very few and short MTs remain. (G,K) Nuclear region (projection from 28.5 to 33 µm deep inside the zygote). (G) The tubulin has not been well incorporated in the nuclear area, which is only faintly labelled. (H,L) Nuclear area from another zygote before (H) and after (L) nocodazole incubation. The bright patches on the left of zygotes in A, E, I, correspond to unincorporated tubulin on the zygote surface near the injection site. Bar, 15 µm.

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