JCS02402 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 (Adobe PDF) -

  Fig. S1. Multiple alignments of SYCE1 (A) and CESC1 (B) orthologues. Light grey shading represents 60%, dark grey shading 80% and black shading 100% conservation.

• Supplemental Figure 2 (Adobe PDF) -

  Fig. S2. SYCE1 and CESC1 localise to the synapsed areas of the homologous chromosomes in mouse oocytes. Meiotic spread-preparations from newborn ovaries were stained with anti-STAG3 (green) and anti-SYCE1 (red), or anti-STAG3 (green) and anti-CESC1 (red). (A-C) Pachytene cell with STAG3 (A), SYCE1 (B) and merged antibody signals and DAPI staining of DNA (C). (D-F) Diplotene cell with STAG3 (D), SYCE1 (E) and merged antibody signals and DAPI staining of DNA (F). (G-I) Pachytene cell with STAG3 (G) CESC1 (H) and merged antibody signals and DAPI staining of DNA (I). (J-L) Diplotene cell with STAG3 (J) CESC1 (K) and merged antibody signals and with DAPI staining of DNA (L). Insets show regions of diplotene spreads with unsynapsed axes that are not stained by antibodies to SYCE1 or CESC1. Bars, 10 mm.