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Fig. 1. Tissue- and cell-specific expression pattern of SYCE1 and CESC1. (A) A multiple-tissue RNA blot was hybridised sequentially with Syce1, Cesc1 and ribosomal protein S26 (Vincent et al., 1993) probes. The signals for Syce1 and Cesc1 are at the sizes predicted for these mRNAs based on sequence databases, and are restricted to testis at this level of detection. (B-M) Frozen testis sections stained with affinity-purified anti-SYCE1 antibody (B,C), affinity-purified anti-SYCE1 antibody competed with immunizing peptide (D), anti-CESC1 IgG peak (H,I) and anti-CESC1 IgG peak competed with GST-CESC1 fusion protein (J). SYCE1 and CESC1 specifically localise to meiotic cells and in particular, to the synaptonemal complex. The same sections were stained for DNA with Hoechst 33258 (E-G, K-M). Bars are 50 µm (B,D,E,G,H,J,K,M) and 10 µm (C,F,I,L).





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