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Fig. 2. SYCE1 and CESC1 localise to the synapsed areas of the homologous chromosomes in mouse spermatocytes. Meiotic spread preparations from adult males were stained with anti-STAG3 (green) and anti-SYCE1 (red), anti-STAG3 (green) and anti-CESC1 (red) or anti-SYCP1 (green) and anti-SYCE1 (red). (A-C) Pachytene cell with STAG3 (A), SYCE1 (B) and merged antibody signals and DAPI staining of DNA (C). (D-F) Diplotene cell with STAG3 (D), SYCE1 (E) and merged antibody signals and DAPI staining of DNA (F). Insets show lack of staining with SYCE1 (arrowheads) of unsynapsed axes. (G-I) Diplotene cell with SYCP1 (G) SYCE1 (H) and merged antibody signals and DAPI staining of DNA (I). (J-L) Pachytene cell with STAG3 (J) CESC1 (K) and merged antibody signals and DAPI DNA staining (L). (M-O) Diplotene cell with STAG3 (M) CESC1 (N) and merged antibody signals and DAPI DNA staining (O). Insets show lack of staining with CESC1 (arrowheads) of unsynapsed axes. (P,Q) Electron microscopy images show the localisation of SYCE1 and CESC1 in relation to the central element (CE) using immuno-gold-labelled anti-SYCE1 and anti-CESC1 antibodies and electron microscopy. Bars, 7.5 µm (A-O) and 0.1 µm (P,Q).





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