JCS02403 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 (Adobe PDF) -

  Fig. S1. MhAT3F cells are partially polarized in monolayer cultures. Cells were grown either in monolayer or included in Matrigel, fixed and analysed. Under both conditions cells had an epithelial morphology with cortical actin distribution and membrane staining of g-catenin. Bars, 20 mm.

• Supplemental Figure 2 (Adobe PDF) -

  Fig. S2. Confluence has no effect on the sensitivity of mhAT3F to Fas stimulation. Cells were seeded as monolayer cultures at 40,000 cm^–2 or 100,000 cm^–2 and grown for 24 hours. The treatment was as indicated and lasted for 22 hours. (A) Cell cultures were examined by phase-contrast microscopy (top) or harvested and centrifuged onto microscope slides after pooling the floating and adherent cells (bottom). The samples were fixed, permeabilized and labelled with Hoechst 33258 (blue) and antibody against active caspase 3 (green). Quantitative estimate of active caspase-3-containing cells in subconfluent (B) and confluent (C) cultures. A minimum of 300 cells were counted per sample in three experiments. Bars, 20 mm.

• Supplemental Figure 3 (Adobe PDF) -

  Fig. S3. Matrigel-grown organoids contain intercellular lumens. Low-power transmission electron microscopy image of an organoid. Bars, 1 mm.