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Fig. 3. mhAT3F organoids are functional. (A) Western-blot analysis of albumin secretion by mhAT3F cells grown under the indicated conditions. Albumin accumulation was allowed to proceed for 24 hours and the amount of medium assayed was normalized to the number of cells present in the culture, estimated by their GAPDH content. A representative blot is shown, the numbers represent mean fold increase compared with monolayer cultures, as calculated after scanning of three western blots. (B) Monolayer grown mhAT3F show no detectable bilirubin (a), whereas the green Hall's staining of the whole organoids (b) indicates the accumulation of bilirubin. A section of a cholestatic human liver (c) is shown as a positive control. Bars. 20 µm.
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