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Fig. 4. Expression of SIAla21, SIAla22, SIAla31, SIAla32, SIAla33 and SIAla41 in COS-1 cells at 37°C and 20°. COS-1 cells were transiently transfected with pSIAla21, pSIAla22, pSIAla31, pSIAla32, pSIAla33 or pSIAla41, labelled and processed in a pulse-chase experiment with [35S]methionine as described for Fig. 2. The deglycosylated and control samples were subjected to SDS-PAGE prior to scanning by a phosphorimaging device. For some gels longer exposure was used because of the faint band intensity.





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