First published online June 23, 2005
Journal of Cell Science 118, 1305e (2005)
© The Company of Biologists Limited
Pathways for podosome patterning
Normal bone is continually resorbed and reformed to maintain its strength. Osteoclasts, the cells that degrade the mineralized bone matrix, attach to bone through a sealing zone within which the matrix is degraded. In vitro, this sealing zone comprises a belt of podosomes (specialized adhesion structures containing P-actin and focal adhesion proteins) that forms during osteoclast maturation and is stabilized by microtubules. Pierre Jurdic and co-workers now report that a novel Rho-mDia2-HDAC6 pathway controls podosome patterning in vitro through acetylation of microtubules (see p. 2901). They show that inhibition of the small GTPase Rho induces the formation of stable, highly acetylated microtubules but that microinjection of Rho or its activated effector mDia2 into osteoclasts disrupts the podosome belt and decreases microtubule acetylation. In addition, mDia2 co-immuniprecipitates with the deacetylase HDAC6 and stimulates its ability to deacetylate microtubules. Furthermore, inhibiting Rho during the early stages of osteoclast differentiation accelerates microtubule acetylation and stabilization of the podosome belt. Pierre Jurdic and co-workers therefore conclude that Rho regulates osteoclast maturation through mDia2 and HDAC6 by affecting microtubule stability and acetylation and actin organization.
Related articles in JCS:
- A novel Rho-mDia2-HDAC6 pathway controls podosome patterning through microtubule acetylation in osteoclasts
- Olivier Destaing, Frédéric Saltel, Benoit Gilquin, Anne Chabadel, Saadi Khochbin, Stéphane Ory, and Pierre Jurdic
JCS 2005 118: 2901-2911.
[Abstract]
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