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Fig. 3. START-containing proteins have distinct subcellular localization. (A) Cotransfection of HeLa cells with StAR (green) and MLN64 (red). MLN64 and StAR show a vesicular staining pattern corresponding to endosomes and mitochondria, respectively. (B) Cotransfection of HeLa cells with MLN64 (red) and GFP-STARD4 (green). MLN64 shows a typical punctate staining corresponding to endosomes whereas the GFP-STARD4 fusion protein gives a diffuse nuclear and cytoplasmic signal. Nuclei were counterstained with Hoechst-33258 dye.





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