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Fig. 7. CNBD is required for ER exit but not tetramer assembly. (A) Cells producing HCN2_wt were treated with PK (+) or left untreated (–) and subjected to immunoblotting with an anti-Myc antibody. PK concentrations are shown on top of each lane. (B) Cells producing HCN2 mutant variants were lysed and subjected to immunoblotting with an anti-Myc antibody. (C) Cells producing HERG_wt and HERG_750-870 were lysed and fractionated on linear non-denaturing sucrose gradients, and samples from the indicated fractions were subjected to immunoblot analysis. Pixel intensities of signals were measured and normalized to the maximum signal intensity. HERG_wt and HERG_750-870 are indicated by ${blacksquare}$ and ${square}$ , respectively. The position of markers is shown by arrows (alcohol dehydrogenase and thyroglobulin, 150 kDa and 669 kDa, respectively). (D) Sucrose-gradient analysis of HCN2 as described for HERG in C.

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