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Fig. 2. Transformation of Paramecium cells with c-subunit-GFP (V0) constructs. Staining with the constructs c1-GFP, c4-GFP and c5-GFP showed no differences; thus, all the c-GFPs seem to be targeted to the same organelles. (a) c5-GFP, strong labeling of the radial canals (rc) of the contractile vacuole complex (cvc); (b) c4-GFP, trichocysts (tr) attached to the cortex are labeled but somewhat obscured by strong cytoplasmic labeling; (c) c4-GFP, the membrane of a food vacuole (fv) is labeled; (d) c4-GFP, when focused on the cell cortex, a regular network is visible, probably representing the ER. Bars, 10 µm.





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