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Fig. 3. GFP localization of the F-subunits of V1. (a) Injection of a moderate amount of F-GFP plasmid into the macronucleus resulted in bright staining of the radial canals (rc) of the contractile vacuole complex; (b) injection of a large amount additionally stained trichocysts (tr), a regular network at the cell cortex, and it caused considerable cytosolic background. Bar, 10 µm.