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Fig. 5. Application of an anti-GFP antibody to living cells transformed simultaneously with, c1-, c4- and c5-GFP constructs. Food vacuole (fv) membranes are stained whereas the cell surface is devoid of any label; (a) GFP labeling by c-GFP fusion genes; (b) anti-GFP antibody, detected with an Alexa-coupled secondary antibody; (c) untransformed control cell; (d) anti-GFP antibody and secondary antibody applied on a control cell show neither cell-surface labeling nor staining of food vacuole membranes. Bar, 10 µm.





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